Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. In simpler terms, a mass spectrummeasures the masses within a sample. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.MALDI TOF/ TOF and ESI mass spectrometry are soft methodes for proteins and macro molecules. Also, Mass spectrometry coupled with GC or HPLC chromatography.