Analytical Laboratory of Medicinal Plants and Drug Research Institute

High-performance liquid chromatography(HPLC; formerly referred to as high-pressure liquid chromatography) is a technique in analytical chemistry  used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out of the column